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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has actually been observed in many tumors, which leads to chromosome instability, and thus may perhaps lead to tumorigenesis. This protein was discovered to affiliate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in selling mobile-cycle development within the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinctive isoforms, have already been explained. Two further splice variants ended up documented but specific nucleotide sequence information will not be nevertheless accessible.
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Blocking peptides are peptides that bind exclusively to the goal antibody and block antibody binding. These peptide ordinarily consists of the epitope identified by the antibody.
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This tutorial outlines even more achievable optimization of cellular and immunoassay parameters to be certain the very best final results are...
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This tutorial outlines even further probable optimization of mobile and immunoassay parameters to be certain the very best success are...
Antibodies sure to the blocking peptide now not bind into the epitope over the concentrate on protein. By evaluating the staining with the blocked antibody vs . the antibody alone, you can see which staining is distinct.
Target Details Cyclin E belongs for the very conserved cyclin family members, whose associates are characterised by a extraordinary periodicity in protein abundance through the cell cycle. Cyclins functionality as regulators of CDK kinases. Unique cyclins exhibit distinct expression and degradation styles which lead towards the temporal coordination of each and every mitotic celebration. Cyclin E kinds a complex with and capabilities being a regulatory check here subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates with the G1-S stage boundary and is particularly degraded as cells development via S section.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by each antibodies...
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